| Date: | 27 September–3 October 2021 |
| Edition: | 3rd |
| Surface: | Hard |
| Location: | Berkeley, California, United States |
| Category: | ITF Women's World Tennis Tour |
| Prize Money: | $60,000 |
| Champs: |  Usue Maitane Arconada |
| Champd: | Sophie Chang / Angela Kulikov |
| Before Year: | 2019 |
The 2021 Berkeley Tennis Club Challenge was a professional women's tennis tournament played on outdoor hard courts. It was the third edition of the tournament which was part of the 2021 ITF Women's World Tennis Tour. It took place in Berkeley, California, United States between 27 September and 3 October 2021.[1]
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 China | Zheng Saisai | 83 | 1 | |
| China | Wang Xinyu | 129 | 2 | |
 Hungary | Panna Udvardy | 143 | 3 | |
 Japan | Kurumi Nara | 176 | 4 | |
| Katie Volynets | 185 | 5 | ||
| Francesca Di Lorenzo | 193 | 6 | ||
| Japan | Mayo Hibi | 209 | 7 | |
| Liang En-shuo | 228 | 8 |
The following players received wildcards into the singles main draw:
Reese Brantmeier Ellie Douglas Victoria Duval
Paola Expósito Díaz DelgadoThe following player received entry using a protected ranking:
The following player received entry as a special exempt:
The following players received entry from the qualifying draw:
Alexandra Bozovic Sophie Chang Jada Hart Haruka Kaji
Maria Kozyreva Hiroko Kuwata Maegan Manasse Emma NavarroSee main article: 2021 Berkeley Tennis Club Challenge – Singles.
Usue Maitane Arconada def. 
Marcela Zacarías, 6–1, 6–3See main article: 2021 Berkeley Tennis Club Challenge – Doubles.
Sophie Chang / Angela Kulikovdef. Liang En-shuo / Lu Jiajing, 6–4, 6–3