| Champ: | Diede de Groot |
| Runner: |  Yui Kamiji |
| Score: | 6–3, 6–7(4–7), 7–6(10–4) |
| Draw: | 8 |
| Seeds: | 2 |
| Before Name: | Australian Open – Wheelchair women's singles |
| After Name: | Australian Open – Wheelchair women's singles |
See main article: 2021 Australian Open.
Diede de Groot defeated the defending champion Yui Kamiji in the final, 6–3, 6–7(4–7), 7–6(10–4) to win the women's singles wheelchair tennis title at the 2021 Australian Open. It was the first step in an eventual Super Slam for de Groot.