| Date: | 7 – 13 June |
| Edition: | 5th |
| Draw: | 32S / 16D |
| Surface: | Clay |
| Location: | Almaty, Kazakhstan |
| Champs: |  Zizou Bergs |
| Champd: | Jesper de Jong /  Vitaliy Sachko |
| Before Year: | 2019 |
| After Year: | 2021 |
| After Name: | Almaty Challenger II |
The 2021 Almaty Challenger was a professional tennis tournament played on clay courts. It was the fifth edition of the tournament which was part of the 2021 ATP Challenger Tour. It took place in Almaty, Kazakhstan between 7 and 13 June 2021.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Slovakia | Andrej Martin | 108 | 1 | |
 Egypt | Mohamed Safwat | 162 | 2 | |
| Belgium | Kimmer Coppejans | 174 | 3 | |
 France | Enzo Couacaud | 177 | 4 | |
 Italy | Lorenzo Giustino | 178 | 5 | |
 Kazakhstan | Dmitry Popko | 195 | 6 | |
 Uzbekistan | Denis Istomin | 204 | 7 | |
 Brazil | João Menezes | 211 | 8 |
The following players received entry into the singles main draw as wildcards:
The following player received entry into the singles main draw as an alternate:
The following players received entry from the qualifying draw:
See main article: 2021 Almaty Challenger – Singles.
Zizou Bergs def. Timofey Skatov 4–6, 6–3, 6–2.See main article: 2021 Almaty Challenger – Doubles.
Vitaliy Sachko def. Vladyslav Manafov / 
Evgenii Tiurnev 7–6(7–4), 6–1.
