The 2020 Prosperita Open was a professional tennis tournament played on clay courts. It was the 17th edition of the tournament which was part of the 2020 ATP Challenger Tour. It took place in Ostrava, Czech Republic between 31 August and 6 September.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Henri Laaksonen | 129 | 1 | ||
 Belarus | Ilya Ivashka | 138 | 2 | |
 Korea, Republic of | Chung Hyeon | 142 | 3 | |
| Ernests Gulbis | 158 | 4 | ||
 Slovakia | Martin Kližan | 160 | 5 | |
 France | ||||
 Canada | Steven Diez | 165 | 7 | |
 Ukraine | Sergiy Stakhovsky | 168 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry into the singles main draw as special exempts:
The following players received entry from the qualifying draw:
The following player received entry as a lucky loser:
Aslan Karatsev def. Oscar Otte 6–4, 6–2.See main article: 2020 Prosperita Open – Doubles.
Artem Sitak / Igor Zelenay def. 
Karol Drzewiecki / Szymon Walków 7–5, 6–4.
