The 2020 Moneta Czech Open was a professional tennis tournament played on clay courts. It was the 27th edition of the tournament which was part of the 2020 ATP Challenger Tour. It took place in Prostějov, Czech Republic between 7–13 September 2020.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Spain | Pablo Andújar | 56 | 1 | |
 Czech Republic | Jiří Veselý | 67 | 2 | |
 Italy | Stefano Travaglia | 87 | 3 | |
| Spain | Jaume Munar | 105 | 4 | |
| Spain | Pedro Martínez | 106 | 5 | |
 Poland | Kamil Majchrzak | 107 | 6 | |
 Japan | Taro Daniel | 112 | 7 | |
 Russian Federation | Evgeny Donskoy | 114 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry into the singles main draw as special exempts:
The following players received entry from the qualifying draw:
The following players received entry as lucky losers:
See main article: 2020 Moneta Czech Open – Singles.
Kamil Majchrzak def. Pablo Andújar 6–2, 7–6(7–5).See main article: 2020 Moneta Czech Open – Doubles.
Zdeněk Kolář / Lukáš Rosol def. 
Sriram Balaji / Divij Sharan 6–2, 2–6, [10–6].
