| Date: | 16–23 November |
| Edition: | 16th |
| Surface: | Clay |
| Location: | Guayaquil, Ecuador |
| Champs: |  Francisco Cerúndolo |
| Champd: |  Luis David Martínez /  Felipe Meligeni Alves |
The 2020 Challenger Ciudad de Guayaquil is a professional tennis tournament played on clay courts. It is the sixteenth edition of the tournament which is part of the 2020 ATP Challenger Tour. It will take place in Guayaquil, Ecuador between 16 and 23 November 2020.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Argentina | Federico Coria | 91 | 1 | |
 Spain | Roberto Carballés Baena | 103 | 2 | |
 Slovakia | Andrej Martin | 105 | 3 | |
| Spain | Jaume Munar | 108 | 4 | |
| Pedro Sousa | 111 | 5 | ||
 Peru | Juan Pablo Varillas | 157 | 6 | |
 Ecuador | Emilio Gómez | 159 | 7 | |
| Alejandro Tabilo | 172 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
See main article: 2020 Challenger Ciudad de Guayaquil – Singles.
Francisco Cerúndolo def. Andrej Martin 6–4, 3–6, 6–2.See main article: 2020 Challenger Ciudad de Guayaquil – Doubles.
Luis David Martínez / Felipe Meligeni Alves def. Sergio Martos Gornés / Jaume Munar 6–0, 4–6, [10–3].