| Champ: |  Yui Kamiji |
| Runner: | Aniek van Koot |
| Score: | 6–2, 6–2 |
| Draw: | 8 |
| Seeds: | 2 |
| Before Name: | Australian Open – Wheelchair women's singles |
| After Name: | Australian Open – Wheelchair women's singles |
See main article: 2020 Australian Open.
Yui Kamiji defeated Aniek van Koot in the final, 6–2, 6–2 to win the women's singles wheelchair tennis title at the 2020 Australian Open.
Diede de Groot was the two-time defending champion, but was defeated by Zhu Zhenzhen in the quarterfinals.