| Date: | 19–25 October |
| Edition: | 33rd |
| Surface: | Hard |
| Location: | Istanbul, Turkey |
| Champs: |  Ilya Ivashka |
| Champd: | Ariel Behar /  Gonzalo Escobar |
The 2020 Amex-Istanbul Challenger was a professional tennis tournament played on hard courts. It was the 33rd edition of the tournament which was part of the 2020 ATP Challenger Tour. It took place in Istanbul, Turkey between 19 and 25 October 2020.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Spain | ||||
| Spain | Jaume Munar | 112 | 2 | |
 Japan | Taro Daniel | 120 | 3 | |
 Russian Federation | Evgeny Donskoy | 121 | 4 | |
| Jason Jung | 127 | 5 | ||
 Italy | Lorenzo Giustino | 140 | 6 | |
| Belarus | Ilya Ivashka | 141 | 7 | |
 Egypt | Mohamed Safwat | 146 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following players received entry as lucky losers:
See main article: 2020 Amex-Istanbul Challenger – Singles.
Ilya Ivashka def. 
Martin Kližan 6–1, 6–4.See main article: 2020 Amex-Istanbul Challenger – Doubles.
Gonzalo Escobar def. 
Robert Galloway / Nathaniel Lammons 4–6, 6–3, [10–7].
