| Date: | 17–23 August 2020 |
| Edition: | 27th |
| Surface: | Clay |
| Location: | Prague, Czech Republic |
| Category: | ATP Challenger Tour |
| Prize Money: | $125,000 |
| Champs: | Stan Wawrinka |
| Champd: |  Pierre-Hugues Herbert / Arthur Rinderknech |
| Before Name: | Advantage Cars Prague Open |
The 2020 I.ČLTK Prague Open is a professional tennis tournament played on outdoor clay courts. It was part of the 2020 ATP Challenger Tour. It took place in Prague, Czech Republic between 17 August and 6 September 2020.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Stan Wawrinka | 17 | 1 | ||
 Czech Republic | Jiří Veselý | 65 | 2 | |
| France | Pierre-Hugues Herbert | 71 | 3 | |
| Philipp Kohlschreiber | 74 | 4 | ||
 Slovakia | Jozef Kovalík | 122 | 5 | |
 India | Sumit Nagal | 127 | 6 | |
| Henri Laaksonen | 137 | 7 | ||
 Belarus | Ilya Ivashka | 138 | 8 | |
| Blaž Rola | 141 | 9 | ||
| Yannick Maden | 149 | 10 | ||
 Belgium | Kimmer Coppejans | 154 | 11 | |
| Slovakia | Martin Kližan | 159 | 12 | |
| France | Arthur Rinderknech | 161 | 13 | |
| Ernests Gulbis | 162 | 14 | ||
 Austria | Sebastian Ofner | 163 | 15 | |
 Canada | Steven Diez | 165 | 16 |
The following players received wildcards into the singles main draw:
The following players received entry into the singles main draw using a protected ranking:
The following players received entry from the qualifying draw:
See main article: 2020 I.ČLTK Prague Open – Singles.
Aslan Karatsev 7–6(7–2), 6–4.See main article: 2020 I.ČLTK Prague Open – Doubles.
Pierre-Hugues Herbert / Arthur Rinderknech def. Zdeněk Kolář / Lukáš Rosol 6–3, 6–4.