| Competition: | Czech Women's First League |
| Season: | 2020–21 |
| Winners: | Sparta Prague |
| Promoted: | none |
| Relegated: | none |
| Continentalcup1: | Champions League |
| Continentalcup1 Qualifiers: | Sparta Prague Slavia Prague Slovácko |
| Matches: | 80 |
| Total Goals: | 346 |
| League Topscorer: | Lucie Martínková (37) |
| Biggest Home Win: | , |
| Biggest Away Win: | , |
| Highest Scoring: | ,,,, |
| Longest Wins: | 13 matches |
| Longest Unbeaten: | 17 matches |
| Longest Winless: | 13 matches |
| Longest Losses: | 6 matches |
| Highest Attendance: | 451 |
| Lowest Attendance: | 20 |
| Prevseason: | 2019–20 |
| Nextseason: | 2021–22 |
| Updated: | 19 June 2021 |
The 2020–21 Czech Women's First League is the 28th season of the Czech Republic's top-tier football league for women. Slavia Prague were the defending champions.
The eight teams played each other twice for a total of 14 matches per team. After that the top four teams played a championship round for another six matches per team. The bottom placed four teams played the relegation round. The champions, runners-up and third-placed teams qualified for the 2021–22 UEFA Women's Champions League.
| Team | Home town | Home ground | Capacity | |
|---|---|---|---|---|
| 2,500 | ||||
| 8,150 | ||||
| Prague | 1,000 | |||
| Městský stadion | ||||
| 8,121 | ||||
| Prague | 20,000 | |||
The regular season ended on 2 May 2021.
Played by the teams placed first to fourth of the regular season. Teams play each other twice.
Played by the teams placed fifth to eighth of the regular season. Teams play each other twice.
Note: Flags indicate national team as has been defined under FIFA eligibility rules. Players may hold more than one non-FIFA nationality.
| Team | Manager | Captain | Kit manufacturer | Shirt sponsor | |
|---|---|---|---|---|---|
| Slovan Liberec |  |  | Nike | Preciosa | |
| Dukla Prague | | | Adidas | ProInterier | |
| Slavia Praha | | | Puma | CITIC Group | |
| Slovácko | | | Puma | Z-Group | |
| Sparta Prague | | | Nike | ||
| Viktoria Plzeň | | | Macron | ||
| Lokomotiva Brno Horní Heršpice | | | Adidas | Exclusia | |
| Pardubice | | |
Final standing
| Rank | Scorer | Club | Goals |
|---|---|---|---|
| 1 | Lucie Martínková | Sparta Prague | 37 |
| 2 | Tereza Kožárová | Slavia Prague | 34 |
| 3 | Aneta Pochmanová | Sparta Prague | 14 |
| 4 | Petra Divišová | Slavia Prague | 11 |
| Kristýna Růžičková | Pardubice | ||
| 5 | Franny Černá | Slavia Prague | 9 |
| 6 | Miroslava Mrázová | Viktoria Plzeň | 8 |
| Markéta Ringelová | Sparta Prague | ||
 Mia Persson | Slavia Prague | ||