The 2019 Winston-Salem Open was a men's tennis tournament played on outdoor hard courts. It was the 51st edition of the Winston-Salem Open (as successor to previous tournaments in New Haven and Long Island), and part of the ATP Tour 250 Series of the 2019 ATP Tour. It took place at Wake Forest University in Winston-Salem, North Carolina, United States, from August 18 through August 24, 2019. It was the last event on the 2019 US Open Series before the 2019 US Open.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 France | 29 | 1 | ||
 Canada | 34 | 2 | ||
 Poland | Hubert Hurkacz | 40 | 3 | |
| 43 | 4 | |||
 United Kingdom | 44 | 5 | ||
| Sam Querrey | 45 | 6 | ||
 Italy | 47 | 7 | ||
 Serbia | 50 | 8 | ||
 Spain | Albert Ramos Viñolas | 51 | 9 | |
| 52 | 10 | |||
| Spain | 53 | 11 | ||
 Norway | Casper Ruud | 54 | 12 | |
| Serbia | 58 | 13 | ||
 Australia | 61 | 14 | ||
| France | 62 | 15 | ||
| Spain | 63 | 16 |
The following players received wildcards into the singles main draw:
The following players received entry using a protected ranking into the singles main draw:
The following players received entry from the qualifying draw:
Nikoloz Basilashvili → replaced by 
Thiago Monteiro Albert Ramos Viñolas
Tennys Sandgren
Federico Delbonis → replaced by Alexei Popyrin
Hugo Dellien → replaced by 
Prajnesh Gunneswaran Bradley Klahn → replaced by Jaume Munar
Jozef Kovalík → replaced by 
Amir Weintraub
Mikhail Kukushkin → replaced by 
Lee Duck-hee Adrian Mannarino → replaced by Denis Kudla
Yoshihito Nishioka → replaced by Antoine Hoang Alexander Bublik ((Injury)) Jérémy Chardy (Ankle injury) Filip Krajinović ((Illness)) Feliciano López ((Unknown))
Lloyd Harris ((Unknown))| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| Poland | Łukasz Kubot | Brazil | Marcelo Melo | 9 | 1 | |
| Rajeev Ram | United Kingdom | Joe Salisbury | 40 | 2 | ||
| Nikola Mektić | Franko Škugor | 43 | 3 | |||
| Kevin Krawietz | Andreas Mies | 43 | 4 |
The following pairs received wildcards into the doubles main draw:
The following pair received entry as alternates:
See main article: 2019 Winston-Salem Open – Singles.
Hubert Hurkacz def. Benoît Paire, 6–3, 3–6, 6–3See main article: 2019 Winston-Salem Open – Doubles.
Łukasz Kubot / Marcelo Melo def. Nicholas Monroe / Tennys Sandgren, 6–7(6–8), 6–1, [10–3]
