| Date: | 13–19 May |
| Edition: | 23rd |
| Draw: | 48S / 16D |
| Surface: | Clay |
| Location: | Samarkand, Uzbekistan |
| Champs: |  João Menezes |
| Champd: | Gonçalo Oliveira /  Andrei Vasilevski |
The 2019 Samarkand Challenger was a professional tennis tournament played on clay courts. It was the 23rd edition of the tournament which was part of the 2019 ATP Challenger Tour. It took place in Samarkand, Uzbekistan between 13 and 19 May 2019.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 France | Corentin Moutet | 122 | 1 | |
| Belarus | Egor Gerasimov | 132 | 2 | |
 Slovakia | Andrej Martin | 206 | 3 | |
 Kazakhstan | Aleksandr Nedovyesov | 209 | 4 | |
 Austria | Lucas Miedler | 233 | 5 | |
| Belarus | Uladzimir Ignatik | 244 | 6 | |
| Gonçalo Oliveira | 259 | 7 | ||
| Brazil | Guilherme Clezar | 273 | 8 | |
 Dominican Republic | José Hernández-Fernández | 288 | 9 | |
| Ulises Blanch | 290 | 10 | ||
 Russian Federation | Roman Safiullin | 293 | 11 | |
 Spain | Roberto Ortega Olmedo | 295 | 12 | |
| Russian Federation | Pavel Kotov | 301 | 13 | |
| Dominican Republic | Roberto Cid Subervi | 302 | 14 | |
 India | Sumit Nagal | 303 | 15 | |
| Marcelo Arévalo | 309 | 16 |
The following players received wildcards into the singles main draw:
Sergey Fomin Andrey Golubev Temur Ismailov Olimjon Nabiev Dmitry PopkoThe following players received entry into the singles main draw using their ITF World Tennis Ranking:
The following player received entry into the singles main draw as an alternate:
Fabrizio OrnagoThe following players received entry from the qualifying draw:
See main article: 2019 Samarkand Challenger – Singles.
João Menezes def. Corentin Moutet 7–6(7–2), 7–6(9–7).See main article: 2019 Samarkand Challenger – Doubles.
Andrei Vasilevski def. Sergey Fomin / Teymuraz Gabashvili 3–6, 6–3, [10–4].
