| Date: | 28 October – 3 November |
| Edition: | 15th |
| Surface: | Clay |
| Location: | Guayaquil, Ecuador |
| Champs: |  Thiago Seyboth Wild |
| Champd: | Ariel Behar /  Gonzalo Escobar |
The 2019 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the fifteenth edition of the tournament which was part of the 2019 ATP Challenger Tour. It took place in Guayaquil, Ecuador between October 28 and November 3, 2019.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Bolivia, Plurination;al State of | Hugo Dellien | 78 | 1 | |
 Argentina | Leonardo Mayer | 95 | 2 | |
 Spain | Jaume Munar | 96 | 3 | |
| Brazil | Thiago Monteiro | 97 | 4 | |
 Slovakia | Andrej Martin | 115 | 5 | |
| Pedro Sousa | 116 | 6 | ||
 Italy | Paolo Lorenzi | 120 | 7 | |
 India | ||||
| Argentina | Federico Coria | 131 | 9 | |
| Ecuador | Emilio Gómez | 143 | 10 | |
| Argentina | Facundo Bagnis | 146 | 11 | |
 Peru | Juan Pablo Varillas | 161 | 12 | |
| Slovakia | Jozef Kovalík | 178 | 13 | |
| Spain | Mario Vilella Martínez | 179 | 14 | |
| Yannick Hanfmann | 187 | 15 | ||
| 188 | 16 |
The following players received wildcards into the singles main draw:
Hugo Dellien Facundo Díaz Acosta
Alejandro González Antonio Cayetano March Alexander ZederbauerThe following player received entry into the singles main draw as an alternate:
Wilson LeiteThe following players received entry from the qualifying draw:
The following player received entry as a lucky loser:
See main article: 2019 Challenger Ciudad de Guayaquil – Singles.
Thiago Seyboth Wild def. Hugo Dellien 6–4, 6–0.See main article: 2019 Challenger Ciudad de Guayaquil – Doubles.
Gonzalo Escobar def. Pedro Sakamoto / Thiago Seyboth Wild 7–6(7–4), 7–6(7–5).