The 2018 Open Città della Disfida was a professional tennis tournament played on clay courts. It was the 19th edition of the tournament which was part of the 2018 ATP Challenger Tour. It took place in Barletta, Italy between 9 and 15 April 2018.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Italy | Marco Cecchinato | 99 | 1 | |
| Gastão Elias | 106 | 2 | ||
| Italy | Stefano Travaglia | 109 | 3 | |
| Pedro Sousa | 117 | 4 | ||
| Yannick Maden | 128 | 5 | ||
 Slovakia | Martin Kližan | 130 | 6 | |
 Spain | Marcel Granollers | 137 | 7 | |
| Slovakia | ||||
| Italy | Lorenzo Sonego | 158 | 9 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following players received entry as lucky losers:
See main article: 2018 Open Città della Disfida – Singles.
Marco Trungelliti def. Simone Bolelli 2–6, 7–6(7–4), 6–4.See main article: 2018 Open Città della Disfida – Doubles.
Denys Molchanov / Igor Zelenay def. Ariel Behar / Máximo González 6–1, 6–2.
