The 2018 Moneta Czech Open was a professional tennis tournament played on clay courts. It was the 25th edition of the tournament which was part of the 2018 ATP Challenger Tour. It took place in Prostějov, Czech Republic between 4–9 June 2018.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Spain | Guillermo García López | 67 | 1 | |
| Pablo Cuevas | 75 | 2 | ||
| Spain | Roberto Carballés Baena | 76 | 3 | |
 Argentina | Guido Pella | 78 | 4 | |
 Czech Republic | Jiří Veselý | 80 | 5 | |
 Bosnia and Herzegovina | Mirza Bašić | 81 | 6 | |
 Kazakhstan | Mikhail Kukushkin | 83 | 7 | |
 Moldova, Republic of | Radu Albot | 97 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry into the singles main draw as alternates:
The following players received entry from the qualifying draw:
The following player received entry as a lucky loser:
Marek JaloviecSee main article: 2018 Moneta Czech Open – Singles.
Jaume Munar def. 
Laslo Đere 6–1, 6–3.See main article: 2018 Moneta Czech Open – Doubles.
Denys Molchanov / 
Igor Zelenay def. Martín Cuevas / Pablo Cuevas 4–6, 6–3, [10–7].
