| Date: | 16–21 October |
| Edition: | 10th |
| Surface: | Clay |
| Location: | Cali, Colombia |
| Champs: |  Federico Delbonis |
| Champd: | Marcelo Arévalo /  Miguel Ángel Reyes-Varela |
The 2017 Milo Open Cali was a professional tennis tournament played on clay courts. It was the tenth edition of the tournament which was part of the 2017 ATP Challenger Tour. It took place in Cali, Colombia between 16 and 21 October 2017.
| Country | Player | Rank | Seed | |
|---|---|---|---|---|
| Argentina | Horacio Zeballos | 67 | 1 | |
| Argentina | Federico Delbonis | 70 | 2 | |
 Dominican Republic | Víctor Estrella Burgos | 78 | 3 | |
 Spain | Roberto Carballés Baena | 103 | 4 | |
 Austria | Gerald Melzer | 156 | 5 | |
 Slovakia | Jozef Kovalík | 167 | 6 | |
| Spain | Ricardo Ojeda Lara | 171 | 7 | |
| Slovakia | Andrej Martin | 177 | 8 |
The following players received wildcards into the singles main draw:
Charles Force
Alejandro González Cristian Rodríguez Luis ValeroThe following players received entry from the qualifying draw:
See main article: 2017 Milo Open Cali – Singles.
Federico Delbonis def. 
Guilherme Clezar 7–6(12–10), 7–5.See main article: 2017 Milo Open Cali – Doubles.
Miguel Ángel Reyes-Varela def. 
Sergio Galdós / Fabrício Neis 6–3, 6–4.