| Champ: |  Petra Kvitová |
| Runner: |  Dominika Cibulková |
| Score: | 6–1, 6–1 |
| Type: | singles doubles |
| Before Name: | Wuhan Open – Singles |
| After Name: | Wuhan Open – Singles |
See main article: article and 2016 Wuhan Open.
Petra Kvitová defeated Dominika Cibulková in the final, 6–1, 6–1 to win the singles tennis title at the 2016 Wuhan Open. It was her second Wuhan Open title.
Venus Williams was the defending champion, but lost in the third round to Svetlana Kuznetsova.
The top eight seeds received a bye into the second round.