| Champ: |  Pablo Carreño Busta |
| Runner: | Roberto Bautista Agut |
| Score: | 6–7(6–8), 7–6(7–1), 6–4 |
| Draw: | 48 (16) |
| Seeds: | 16 |
| Type: | singles doubles |
See main article: article and 2016 Winston-Salem Open. Kevin Anderson was the defending champion, but lost in the second round to Jiří Veselý.
Pablo Carreño Busta won his maiden ATP title, defeating Roberto Bautista Agut in the final, 6–7(6–8), 7–6(7–1), 6–4.
All seeds receive a bye into the second round.