| O'zbekiston Kubogi 2016 | |
| Champions: | Lokomotiv Tashkent |
| Runner-Up: | Nasaf Qarshi |
| Prevseason: | 2015 |
| Nextseason: | 2017 |
The 2016 Uzbekistan Cup is the 24th season of the annual Uzbek football Cup competition. The competition started on 14 March 2016 and ended in November 2016.The cup winner is guaranteed a place in the 2017 AFC Champions League.
Kokand advance 2–1 on aggregateMetallurg Bekabad advance 4–2 on aggregateMash'al Mubarek advance 2–1 on aggregateAndijan advance 5–3 on aggregateBunyodkor advance 4–3 on aggregateNasaf advance 5–1 on aggregateBukhoro advance 2–0 on aggregateLokomotiv Tashkent advance 13–1 on aggregate
FK Buxoro advance 3–1 on aggregateNasaf Qarshi advance 4–1 on aggregateLokomotiv Tashkent advance 5–3 on aggregate
Bunyodkor advance 3–1 on aggregate
Nasaf Qarshi advance 4–1 on aggregateLokomotiv Tashkent advance 2–0 on aggregate