| Date: | 14 November – 20 November |
| Edition: | 12th |
| Surface: | Clay |
| Location: | Montevideo, Uruguay |
| Champs: |  Diego Schwartzman |
| Champd: | Andrés Molteni / Diego Schwartzman |
The 2016 Uruguay Open is a professional tennis tournament played on clay courts. It is the twelfth edition of the tournament which is part of the 2016 ATP Challenger Tour. It takes place in Montevideo, Uruguay between November 14 and November 20, 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Spain | Nicolás Almagro | 45 | 1 | |
| Argentina | Diego Schwartzman | 58 | 2 | |
| Argentina | Horacio Zeballos | 81 | 3 | |
| Spain | Íñigo Cervantes | 83 | 4 | |
| Argentina | Carlos Berlocq | 89 | 5 | |
 Brazil | Rogério Dutra Silva | 106 | 6 | |
| Argentina | Guido Andreozzi | 109 | 7 | |
| Argentina | Nicolás Kicker | 110 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
See main article: 2016 Uruguay Open – Singles.
Diego Schwartzman def. Rogério Dutra Silva, 6–4, 6–1.[1]See main article: 2016 Uruguay Open – Doubles.
Andrés Molteni / Diego Schwartzman def. Fabiano de Paula / Cristian Garín, walkover.