| Champ: |  Juan Mónaco |
| Runner: |  Jack Sock |
| Score: | 3–6, 6–3, 7–5 |
| Type: | singles doubles |
See main article: 2016 U.S. Men's Clay Court Championships. Jack Sock was the defending champion, but lost in the final to Juan Mónaco, 6–3, 3–6, 5–7.
The top four seeds receive a bye into the second round.