The 2016 U.S. Men's Clay Court Championships (also known as the Fayez Sarofim & Co. U.S. Men's Clay Court Championships for sponsorship purposes) was a tennis tournament played on outdoor clay courts. It was the 48th edition of the U.S. Men's Clay Court Championships, and an ATP World Tour 250 event on the 2016 ATP World Tour. It took place at River Oaks Country Club in Houston, Texas, United States, from April 4 through April 10, 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| John Isner | 13 | 1 | ||
 France | Benoît Paire | 22 | 2 | |
 Spain | Feliciano López | 23 | 3 | |
| Jack Sock | 24 | 4 | ||
| Sam Querrey | 34 | 5 | ||
| Steve Johnson | 36 | 6 | ||
 Cyprus | Marcos Baghdatis | 40 | 7 | |
 Italy | Paolo Lorenzi | 54 | 8 |
The following players received wildcards into the main draw:
The following player received entry with a protected ranking:
The following players received entry via the qualifying draw:
The following player received entry as a lucky loser:
Dmitry Tursunov
Mikhail Kukushkin → replaced by 
Lukáš Lacko
Leonardo Mayer → replaced by Benjamin Becker
John Millman → replaced by 
Reilly Opelka| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| Bob Bryan | Mike Bryan | 11 | 1 | |||
 Austria | Alexander Peya | Philipp Petzschner | 63 | 2 | ||
| Eric Butorac | Scott Lipsky | 89 | 3 | |||
| Steve Johnson | Sam Querrey | 90 | 4 |
The following pairs received wildcards into the doubles main draw:
The following pair received entry as alternates:
See main article: 2016 U.S. Men's Clay Court Championships – Singles.
Juan Mónaco defeated Jack Sock, 3–6, 6–3, 7–5See main article: 2016 U.S. Men's Clay Court Championships – Doubles.
Bob Bryan / Mike Bryan defeated 
Víctor Estrella Burgos / 
Santiago González, 4–6, 6–3, [10–8]