| Date: | 14 November – 20 November |
| Edition: | 3rd |
| Surface: | Carpet / Indoor |
| Location: | Brescia, Italy |
| Champs: |  Luca Vanni |
| Champd: |  Mikhail Elgin / Alexander Kudryavtsev |
The 2016 Trofeo Città di Brescia is a professional tennis tournament played on carpet courts. It is the third edition of the tournament which was part of the 2016 ATP Challenger Tour. It takes place in Brescia, Italy between November 14 and November 20, 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Slovakia | Lukáš Lacko | 112 | 1 | |
 Czech Republic | ||||
| Marco Chiudinelli | 116 | 3 | ||
| Russian Federation | Evgeny Donskoy | 118 | 4 | |
 Argentina | Leonardo Mayer | 132 | 5 | |
 Spain | Enrique López Pérez | 163 | 6 | |
| Russian Federation | Alexander Kudryavtsev | 173 | 7 | |
 Serbia | Marko Tepavac | 175 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw with a protected ranking:
The following player received entry into the singles main draw as a special exempt:
The following players received entry from the qualifying draw:
Egor Gerasimov Marek Jaloviec Petr MichnevThe following players received entry as lucky losers:
See main article: 2016 Trofeo Città di Brescia – Singles.
Luca Vanni def. 
Laurynas Grigelis, 6–7(5–7), 6–4, 7–6(10–8).See main article: 2016 Trofeo Città di Brescia – Doubles.
Mikhail Elgin / Alexander Kudryavtsev def. Wesley Koolhof / Matwé Middelkoop, 7–6(7–4), 6–3.