| Date: | 28 March – 3 April |
| Edition: | 14th |
| Draw: | 32S / 16D |
| Surface: | Hard |
| Prize Money: | $75,000+H |
| Location: | León, Mexico |
| Champs: | Michael Berrer |
| Champd: |  Santiago González / Mate Pavić |
The 2016 Torneo Internacional Challenger León was a professional tennis tournament played on hard courts. It was the fourteenth edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in León, Mexico between 28 March and 3 April 2015.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Australia | Sam Groth | 77 | 1 | |
| Taylor Fritz | 81 | 2 | ||
 Tunisia | Malek Jaziri | 92 | 3 | |
 Japan | Yūichi Sugita | 97 | 4 | |
| Austin Krajicek | 104 | 5 | ||
| Michael Berrer | 118 | 6 | ||
 France | Stéphane Robert | 119 | 7 | |
| Japan | Tatsuma Ito | 120 | 8 |
The following players received wildcards into the singles main draw:
Lucas Gómez Hans Hach Verdugo Tigre Hank Alejandro Moreno FigueroaThe following players received entry from the qualifying draw:
Emilio Gómez
Robin Staněk
Agustín Velotti
Caio ZampieriThe following players received entry courtesy of a special exempt:
The following players received entry as alternates:
See main article: 2016 Torneo Internacional Challenger León – Singles.
João Souza, 6–3, 6–2See main article: 2016 Torneo Internacional Challenger León – Doubles.
Santiago González / Mate Pavić def. Sam Groth / 
Leander Paes, 6–4, 3–6, [13–11]
