The 2016 Tilia Slovenia Open was a professional tennis tournament played on hard courts. It was the fourth edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Portorož, Slovenia between 8 – 13 August 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Florian Mayer | 98 | 1 | ||
 Argentina | Renzo Olivo | 110 | 2 | |
 Slovakia | Jozef Kovalík | 125 | 3 | |
| Grega Žemlja | 142 | 4 | ||
 Hungary | Márton Fucsovics | 160 | 6 | |
 Russian Federation | Alexander Kudryavtsev | 169 | 7 | |
 Belgium | Ruben Bemelmans | 170 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Daniel Cox
Cem İlkel
Edan Leshem
Vadym UrsuThe following player received entry as a lucky loser:
See main article: 2016 Tilia Slovenia Open – Singles.
Daniil Medvedev, 6–1, 6–2See main article: 2016 Tilia Slovenia Open – Doubles.
Sergey Betov / Ilya Ivashka def. Tomislav Draganja / Nino Serdarušić, 1–6, 6–3, [10–4]