| Date: | 26 September – 2 October |
| Edition: | 10th |
| Draw: | 32S / 16D |
| Surface: | Hard |
| Prize Money: | $100,000 |
| Location: | Tiburon, United States |
| Champs: | Darian King |
| Champd: |  Matt Reid / John-Patrick Smith |
The 2016 Tiburon Challenger was a professional tennis tournament played on outdoor hard courts. It was the tenth edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Tiburon, United States between September 26 and October 2, 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Benjamin Becker | 97 | 1 | ||
 United States | Bjorn Fratangelo | 110 | 2 | |
| United States | Tim Smyczek | 112 | 3 | |
| United States | Frances Tiafoe | 122 | 4 | |
| United States | Dennis Novikov | 133 | 5 | |
| United States | Stefan Kozlov | 155 | 6 | |
| United States | Ernesto Escobedo | 139 | 7 | |
 France | Quentin Halys | 143 | 8 |
The following players received wildcards into the singles main draw:
Brian Baker Robbie C. Bellamy Mackenzie McDonald Noah RubinThe following players received entry into the singles main draw as special exempts:
The following players received entry from the qualifying draw:
See main article: article and 2016 Tiburon Challenger – Singles.
Michael Mmoh, 7–6(7–2), 6–2.[1]See main article: article and 2016 Tiburon Challenger – Doubles.
Matt Reid / John-Patrick Smith def. Quentin Halys / Dennis Novikov, 6–1, 6–2.
