The 2016 Thindown Challenger Biella is a professional tennis tournament played on outdoor red clay courts. It is part of the 2016 ATP Challenger Tour. It took place in Biella, Italy between 25 and 31 July 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Italy | Paolo Lorenzi | 48 | 1 | |
 Brazil | Thomaz Bellucci | 49 | 2 | |
 Serbia | Dušan Lajović | 83 | 3 | |
 Argentina | Carlos Berlocq | 87 | 4 | |
 Russian Federation | Karen Khachanov | 104 | 5 | |
 Slovakia | Andrej Martin | 124 | 6 | |
| Argentina | Marco Trungelliti | 130 | 7 | |
| Daniel Brands | 135 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw as a special exempt:
The following players received entry from the qualifying draw:
Tomislav Brkić Andrea Collarini Francisco BahamondeSee main article: 2016 Thindown Challenger Biella – Singles.
Federico Gaio def. Thomaz Bellucci 7–6(7–5), 6–2.See main article: 2016 Thindown Challenger Biella – Doubles.
Leander Paes def. Andrej Martin / Hans Podlipnik 6–4, 6–4.