| Season: | 2016 |
| League Topscorer: | Sarawut Choenchai (15 Goals) |
| Biggest Home Win: | Sa Kaeo 10–0 TA Benchamarachuthit (8 May 2016) |
| Biggest Away Win: | TA Benchamarachuthit 0–5 Pattaya City (19 March 2016) |
| Highest Scoring: | Sa Kaeo 10–0 TA Benchamarachuthit (8 May 2016) |
| Matches: | 132 |
| Total Goals: | 360 |
| Highest Attendance: | 2,206 Cha Choeng Sao 0–1 Sa Kaeo (1 May 2016) |
| Lowest Attendance: | 32 Pattaya City 0–1 Nakhon Nayok (22 May 2016) |
| Nextseason: | (T4)2017 |
2016 Regional League Division 2 Eastern Region is the 8th season of the League competition since its establishment in 2009. It is in the third tier of the Thai football league system.
Nakhon Nayok, Prachinburi United, Kabin United, Sa Kaeo, Marines Maptaphut, Chanthaburi and Samut Prakan Songsingh are broken from Central & Eastern Region
Rayong were promoted to the 2016 Thai Division 1 League.
TA Benchamarachuthit joined the newly expanded league setup.
| Team | Location | Stadium | Capacity | |
|---|---|---|---|---|
| Cha Choeng Sao | Chachoengsao | Chachoengsao Town municipality Stadium | 6,000 | [1] |
| Chanthaburi | Chanthaburi | Chanthaburi Province Stadium | 4,800 | [2] |
| Saimitr Kabin United | Prachinburi | Nom Klao Maharaj Stadium | 3,000 | [3] |
| Marines Eureka | Rayong | Klaeng Municipality Stadium | ? | [4] |
| Nakhon Nayok | Nakhon Nayok | Nakhon Nayok Provincial Administrative Organization Stadium | 2,406 | [5] |
| Prachinburi United | Prachinburi | 3,000 | [6] | |
| Nong Prue 1 StadiumNong Prue 2 Stadium Planet Football Stadium | 6,000? ? | [7] | ||
| Pluak Daeng Rayong United | Rayong | Rayong Province Central Stadium | 4,512 | [8] |
| Sa Kaeo | Sa Kaeo | Sa Kaeo Provincial Administrative Organization Stadium | ? | [9] |
| Samut Prakan | Samut Prakan | 4,100 | [10] | |
| TA Benchamarachuthit | Chanthaburi | Rambhai Barni Rajabhat University Stadium | 2,000 | [11] |
| Trat | Trat | Trat Province Stadium | 5,000 | [12] |
As of 4 September 2016.
| Rank | Player | Club | Goals |
|---|---|---|---|
| 1 |  Sarawut Choenchai | Cha Choeng Sao | 15 |
| 2 |  Erivaldo Oliveira | Trat | 11 |
| 3 |  Soma Otani | Pattaya City | 10 |
| Jirawut Saranan | Trat | ||
| 5 | Kritsada Naknoun | Prachinburi United | 9 |
| Jaruwat Nammool | Prachinburi United | ||
| 7 | Wuttichai Asusheewa | Trat | 8 |
| Pratya Narach | Rayong United | ||
| Watthana Suknarm | Pattaya City | ||
| 10 | Yu Kuboki | Cha Choeng Sao | 7 |
 Cho Byung-jun | Chanthaburi | ||