| Date: | October 31 – November 6 |
| Edition: | 12th |
| Category: | ITF Women's Circuit |
| Surface: | Hard – indoors |
| Location: | Toronto, Ontario, Canada |
| Venue: | Aviva Centre |
| Champs: |  Catherine Bellis |
| Champd: |  Gabriela Dabrowski / Michaëlla Krajicek |
The 2016 Tevlin Women's Challenger was a professional tennis tournament played on indoor hard courts. It was the 12th edition of the tournament and part of the 2016 ITF Women's Circuit, offering a total of $50,000 in prize money. It took place in Toronto, Ontario, Canada between October 31 and November 6, 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Catherine Bellis | 101 | 1 | ||
 Belgium | Elise Mertens | 132 | 2 | |
| Canada | Françoise Abanda | 169 | 3 | |
 Czech Republic | Jesika Malečková | 220 | 4 | |
 United Kingdom | Laura Robson | 221 | 5 | |
 Serbia | Jovana Jakšić | 224 | 6 | |
| Lauren Albanese | 249 | 7 | ||
| Michaëlla Krajicek | 258 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Emina Bektas Julia Elbaba Katherine Sebov Ronit YurovskyThe following player received entry by junior exempt:
See main article: 2016 Tevlin Women's Challenger – Singles.
Catherine Bellis def. Jesika Malečková, 6–2, 1–6, 6–3See main article: 2016 Tevlin Women's Challenger – Doubles.
Gabriela Dabrowski / Michaëlla Krajicek def. Ashley Weinhold / Caitlin Whoriskey, 6–4, 6–3