| Date: | 7–13 November |
| Edition: | 17th (ATP) 8th (ITF) |
| Category: | ATP Challenger Tour ITF Women's Circuit |
| Prize Money: | €85,000+H (ATP) $25,000 (ITF) |
| Location: | Bratislava, Slovakia |
| Champms: |  Norbert Gombos |
| Champws: |  Andreea Mitu |
| Champmd: |  Ken Skupski / Neal Skupski |
| Champwd: | Jocelyn Rae / Anna Smith |
The 2016 Slovak Open was a professional tennis tournament played on indoor hard courts. It was the 17th edition of the tournament which was part of the 2016 ATP Challenger Tour. It was also the 8th edition of the tournament which was part of the ITF Women's Circuit. It took place in Bratislava, Slovakia between 7 and 13 November 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Florian Mayer | 52 | 1 | ||
 Ukraine | Illya Marchenko | 69 | 2 | |
 Czech Republic | Adam Pavlásek | 83 | 3 | |
| Jan-Lennard Struff | 91 | 4 | ||
| Czech Republic | Lukáš Rosol | 106 | 5 | |
 Russian Federation | Daniil Medvedev | 108 | 6 | |
| Slovakia | Lukáš Lacko | 113 | 7 | |
| Slovakia | Jozef Kovalík | 120 | 8 |
The following players received wildcards into the singles main draw:
Lukáš Klein Tomáš Líška Alex Molčan Patrik NémaThe following player received entry to the main draw using a protected ranking:
The following players received entry from the qualifying draw:
Patrik Fabian Filip Horanský Petr Michnev
Sebastian Ofner| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Czech Republic | Denisa Allertová | 93 | 1 | |
| Slovakia | Rebecca Šramková | 121 | 2 | |
| Isabella Shinikova | 137 | 3 | ||
| Tamara Korpatsch | 165 | 4 | ||
| Arantxa Rus | 176 | 5 | ||
| Czech Republic | Tereza Smitková | 183 | 6 | |
| Romania | Andreea Mitu | 212 | 7 | |
| Czech Republic | Jesika Malečková | 218 | 8 |
The following players received wildcards into the singles main draw:
Magdaléna Pantůčková Kristína Schmiedlová Chantal Škamlová Natália VajdováThe following players received entry from the qualifying draw:
Maja Chwalińska Lenka Juríková Barbora Miklová Anastasia Pribylova
Kimberley ZimmermannSee main article: 2016 Slovak Open – Men's singles.
Norbert Gombos def. Marius Copil, 7–6(10–8), 4–6, 6–3 Andreea Mitu def. Denisa Allertová, 6–2, 6–3See main article: 2016 Slovak Open – Men's doubles.
Ken Skupski / Neal Skupski def. 
Purav Raja / Divij Sharan, 4–6, 6–3, [10–5] Jocelyn Rae / Anna Smith def. Quirine Lemoine / Eva Wacanno, 6–3, 6–2