See main article: 2016 Sarasota Open.
Facundo Argüello and Facundo Bagnis were the defending champions but only Argüello has returned to defend his title partnering Nicolás Kicker.
Argüello and Kicker won the title, defeating Marcelo Arévalo and Sergio Galdós 4–6, 6–4, [10–6] in the final.