The 2016 Sarasota Open was a professional tennis tournament played on clay courts. It was the 8th edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Sarasota, Florida, United States between April 11 and April 17, 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Denis Kudla | 59 | 1 | ||
 Argentina | Diego Schwartzman | 88 | 2 | |
 Austria | Gerald Melzer | 117 | 3 | |
 Georgia | Nikoloz Basilashvili | 121 | 4 | |
| Tim Smyczek | 125 | 5 | ||
| Bjorn Fratangelo | 131 | 6 | ||
| Argentina | Facundo Argüello | 139 | 7 | |
| Jared Donaldson | 142 | 8 |
The following players received wildcards into the singles main draw:
Sekou Bangoura Ernesto Escobedo Connor Smith
Dmitry TursunovThe following players received entry from the qualifying draw:
See main article: 2016 Sarasota Open – Singles.
Gerald Melzer, 6–4, 7–6(7–2)See main article: 2016 Sarasota Open – Doubles.
Facundo Argüello / Nicolás Kicker def. 
Marcelo Arévalo / 
Sergio Galdós, 4–6, 6–4, [10–6]
