| Date: | 17 – 22 October |
| Edition: | 2nd |
| Surface: | Clay |
| Location: | Santiago, Chile |
| Champs: |  Máximo González |
| Champd: | Julio Peralta / Horacio Zeballos |
The 2016 Santiago Challenger was a professional tennis tournament played on clay courts. It was the second edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Santiago, Chile between 17 and 22 October 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Argentina | Horacio Zeballos | 73 | 1 | |
 Brazil | Thiago Monteiro | 88 | 2 | |
| Argentina | ||||
| Brazil | Rogério Dutra Silva | 107 | 4 | |
| Brazil | João Souza | 118 | 5 | |
| Argentina | Guido Andreozzi | 126 | 6 | |
| Argentina | Leonardo Mayer | 127 | 7 | |
| Argentina | Nicolás Kicker | 132 | 8 |
The following players received wildcards into the singles main draw:
Jaume MunarThe following player received entry into the singles main draw using a protected ranking:
The following player entered as an alternate:
The following players received entry from the qualifying draw:
The following players entered as lucky losers:
See main article: 2016 Santiago Challenger – Singles.
Máximo González def. Rogério Dutra Silva, 6–2, 7–6(7–5).See main article: 2016 Santiago Challenger – Doubles.
Horacio Zeballos def. 
Sergio Galdós / Máximo González, 6–3, 6–4.