The 2016 San Luis Open Challenger Tour was a professional tennis tournament played on hard courts. It was the 23rd edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in San Luis Potosí, Mexico between 21 and 27 March.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Michael Berrer | 112 | 1 | ||
 Spain | Albert Montañés | 133 | 2 | |
 Brazil | André Ghem | 154 | 3 | |
| Spain | Adrián Menéndez Maceiras | 159 | 4 | |
 Italy | Matteo Donati | 181 | 5 | |
 France | Mathias Bourgue | 187 | 6 | |
| Hans Podlipnik | 196 | 7 | ||
 Ireland | James McGee | 203 | 8 |
The following players received wildcards into the singles main draw:
Mauricio Astorga Lucas Gómez Manuel Sánchez Tigre HankThe following player received entry as an alternate:
The following players received entry from the qualifying draw:
Robin Staněk
Agustín Velotti Caio ZampieriSee main article: 2016 San Luis Open Challenger Tour – Singles.
Peđa Krstin def. Marcelo Arévalo, 6–4, 6–2See main article: 2016 San Luis Open Challenger Tour – Doubles.
Marcus Daniell / Artem Sitak def. Santiago González / Mate Pavić, 6–3, 7–6(7–4)