| Date: | 14–19 March |
| Edition: | 1st |
| Category: | WTA 125K series |
| Surface: | Hard |
| Prize Money: | $125,000 |
| Location: | San Antonio, United States |
| Champs: |  Misaki Doi |
| Champd: | Anna-Lena Grönefeld /  Nicole Melichar |
The 2016 San Antonio Open was a professional tennis tournament played on outdoor hard courts. It was the 1st edition of the tournament as part of the 2016 WTA 125K series. It took place in San Antonio, United States, on 12–19 March 2016.[1]
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Australia | Daria Gavrilova | 33 | 1 | |
 Romania | Irina-Camelia Begu | 35 | 2 | |
| Jeļena Ostapenko | 40 | 3 | ||
 Belgium | Yanina Wickmayer | 47 | 4 | |
 Brazil | Teliana Pereira | 50 | 5 | |
| Japan | Misaki Doi | 55 | 6 | |
| Belgium | Kirsten Flipkens | 59 | 7 | |
| Japan | Nao Hibino | 60 | 8 |
The following players received wildcards into the singles main draw:
Karin Knapp →replaced by Lauren Davis
Bojana Jovanovski →replaced by Naomi Broady| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| Australia | Casey Dellacqua | Darija Jurak | 46 | 1 | ||
| Chuang Chia-jung |  China | Liang Chen | 72 | 2 | ||
 Poland | Klaudia Jans-Ignacik | Australia | Anastasia Rodionova | 89 | 3 | |
| Anna-Lena Grönefeld | Nicole Melichar | 106 | 4 |
The following pair received a wildcard into the doubles main draw:
See main article: 2016 San Antonio Open – Singles.
Misaki Doi def. Anna-Lena Friedsam, 6–4, 6–2See main article: 2016 San Antonio Open – Doubles.
Nicole Melichar def. Klaudia Jans-Ignacik / Anastasia Rodionova, 6–1, 6–3