| Date: | 18–24 April |
| Edition: | 6th |
| Draw: | 32S / 16D |
| Surface: | Clay |
| Prize Money: | $50,000+H |
| Location: | São Paulo, Brazil |
| Champd: |  Fabrício Neis / Caio Zampieri |
The 2016 São Paulo Challenger de Tênis was a professional tennis tournament played on clay courts. It was the sixth edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in São Paulo, Brazil between 18 and 24 April 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Brazil | André Ghem | 165 | 1 | |
| Brazil | João Souza | 187 | 2 | |
| Brazil | Guilherme Clezar | 194 | 3 | |
| Brazil | Thiago Monteiro | 200 | 4 | |
| Gonzalo Lama | 216 | 5 | ||
 El Salvador | Marcelo Arévalo | 222 | 6 | |
 Sweden | Christian Lindell | 239 | 7 | |
 Argentina | Maximiliano Estévez | 273 | 8 |
The following players received wildcards into the singles main draw:
Gabriel Décamps Rafael Camilo Wilson Leite Felipe Meligeni AlvesThe following players received entry from the qualifying draw:
João Pedro Sorgi Ricardo Hocevar André Miele Alexandre TsuchiyaSee main article: 2016 São Paulo Challenger de Tênis – Singles.
Ernesto Escobedo, 6–2, 6–2See main article: 2016 São Paulo Challenger de Tênis – Doubles.
Fabrício Neis / Caio Zampieri def. José Pereira / Alexandre Tsuchiya, 6–4, 7–6(7–3)