| Champ: |  Simona Halep |
| Runner: |  Madison Keys |
| Score: | 7–6(7–2), 6–3 |
| Draw: | 56 (12 / 3) |
| Seeds: | 16 |
| Before Name: | Rogers Cup – Women's singles |
| After Name: | Rogers Cup – Women's singles |
See main article: 2016 Rogers Cup.
Simona Halep defeated Madison Keys in the final, 7–6(7–2), 6–3 to win the women's singles tennis title at the 2016 Canadian Open.
Belinda Bencic was the reigning champion, but withdrew with a left wrist injury before the tournament.
The top eight seeds received a bye into the second round.