| Champ: |  Carla Suárez Navarro |
| Runner: | Jeļena Ostapenko |
| Score: | 1–6, 6–4, 6–4 |
| Type: | singles doubles |
| Draw: | 56 (8 / 3) |
| Seeds: | 16 |
| Before Name: | Qatar Total Open – Singles |
| After Name: | Qatar Total Open – Singles |
See main article: 2016 Qatar Total Open.
Carla Suárez Navarro defeated Jeļena Ostapenko in the final, 1–6, 6–4, 6–4 to win the singles tennis title at the 2016 WTA Qatar Open.
Lucie Šafářová was the defending champion, but lost to Çağla Büyükakçay in the second round.
The top eight seeds received a bye into the second round.