| President: | David Koch |
| Coach: | Ken Hinkley |
| Captain: | Travis Boak |
| Home Ground: | Adelaide Oval |
| Prevseason: | 2015 |
| Nextseason: | 2017 |
See main article: 2016 AFL season. The 2016 Port Adelaide Football Club season was the club's 146th season since formation, the club's 139th season of senior competition and its 20th season in the Australian Football League (AFL). The club also fielded its reserves team in the SANFL.
The following summarises all player changes between the conclusion of the 2015 season and the beginning of the 2016 season.
| Player | Previous Club | League | via | |
|---|---|---|---|---|
| Jimmy Toumpas | Trade period | |||
| Charlie Dixon | Gold Coast | Australian Football League | Trade period |
| Player | New Club | League | via | |
|---|---|---|---|---|
| Kane Cornes | Retired | |||
| Tom Logan | Retired | Australian Football League | ||
| Daniel Flynn | Retired | Australian Football League | ||
| Mitch Harvey | Delisted | Australian Football League | ||
| Andrew Moore | Delisted | Australian Football League | ||
| Jarrad Redden | Delisted | Australian Football League | ||
| Mason Shaw | Delisted | Australian Football League | ||
| Sam Russell | Delisted | Australian Football League | ||
| Johann Wagner | Delisted | Australian Football League |
Bold indicates home game
| Rd | Day | Date | Time PM | Opponent | Scores (Port Adelaide's scores indicated in bold) | Venue | Attendance | Ladder position | |||
| Home | Away | Result | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Sun | March 27 | 2:50 | 20.13 (133) | 15.10 (100) | Won by 33 | Adelaide Oval | 43,807 | 6th | ||
| 2 | Sat | 2 April | 1:15 | 22.12 (144) | 11.20 (86) | Lost by 58 | Adelaide Oval | 51,585 | 12th | ||
| 3 | Fri | April 8 | 7:20 | 17.10 (112) | 7.9 (51) | Won by 61 | Adelaide Oval | 44,601 | 8th | ||
| 4 | Sun | 17 April | 1:10 | 22.19 (151) | 9.11 (65) | Lost by 86 | Manuka Oval | 10,028 | 11th | ||
| 5 | Sat | April 23 | 7:10 | 8.11 (59) | 16.11 (107) | Lost by 48 | Adelaide Oval | 44,937 | 12th | ||
| 6 | Sat | 30 April | 7:25 | 8.11 (59) | 13.16 (94) | Won by 35 | MCG | 27,077 | 11th | ||
| 7 | Sun | align=left | May 8 | 4:10 | 21.10 (136) | 7.17 (59) | Won by 77 | Adelaide Oval | 32,399 | 10th | |
| 8 | Sun | align=left | 15 May | 1:10 | 14.9 (93) | 13.13 (91) | Lost by 2 | Etihad Stadium | 26,924 | 10th | |
| 9 | Sat | align=left | May 21 | 4:05 | 13.8 (86) | 14.10 (94) | Lost by 8 | Adelaide Oval | 38,695 | 10th | |
| 10 | Sat | align=left | 28 May | 1:40 | 10.16 (76) | 18.13 (121) | Won by 45 | TIO Traeger Park | 5,146 | 10th | |
| 11 | Sun | align=left | 8 June | 1:10 | 7.16 (58) | 19.11 (125) | Won by 67 | MCG | 28,567 | 9th | |
| 12 | Sat | June 11 | 1:10 | 14.13 (97) | 15.10 (100) | Lost by 3 | Adelaide Oval | 40,096 | 9th | ||
| 13 | Sat | 18 June | 2:35 | 12.14 (86) | 9.15 (69) | Lost by 17 | Domain Stadium | 32,448 | 9th | ||
| 14 | Bye | 9th | |||||||||
| 15 | Fri | July 1 | 7:20 | (2) | 14.10 (94) | 8.8 (56) | Won by 38 | Adelaide Oval | 37,848 | 9th | |
| 16 | Thu | July 7 | 7:20 | 12.7 (79) | 15.11 (101) | Lost by 22 | Adelaide Oval | 43,025 | 9th | ||
| 17 | Sat | 16 July | 4:35 | 10.17 (77) | 16.9 (105) | Won by 28 | Etihad Stadium | 24,361 | 9th | ||
| 18 | Sun | July 24 | 4:10 | (2) | 9.6 (60) | 11.13 (79) | Lost by 19 | Adelaide Oval | 27,935 | 10th | |
| 19 | Sat | 30 July | 7:25 | (2) | 11.13 (79) | 25.23 (173) | Won by 94 | The Gabba | 13,085 | 9th | |
| 20 | Sat | 6 August | 1:45 | 14.16 (100) | 4.9 (33) | Lost by 67 | SCG | 30,204 | 10th | ||
| 21 | Sat | August 13 | 7:10 | (2) | 8.6 (54) | 13.16 (94) | Lost by 40 | Adelaide Oval | 33,426 | 11th | |
| 22 | Sat | August 20 | 7:10 | (2) | 14.10 (94) | 15.19 (109) | Lost by 15 | Adelaide Oval | 49,541 | 11th | |
| 23 | Sat | 27 August | 12:00 | 9.12 (66) | 13.11 (89) | Won by 23 | Metricon Stadium | 9,213 | 10th | ||
| Source | |||||||||||