The 2016 Poprad-Tatry ATP Challenger Tour was a professional tennis tournament played on clay courts. It was the second edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Poprad, Slovakia between 13 and 18 June 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Slovakia | Martin Kližan | 51 | 1 | |
 Argentina | Horacio Zeballos | 92 | 2 | |
 Russian Federation | 3 | |||
| Slovakia | Andrej Martin | 110 | 4 | |
 Czech Republic | Adam Pavlásek | 114 | 5 | |
 Austria | Gerald Melzer | 122 | 6 | |
| Slovakia | Jozef Kovalík | 134 | 7 | |
| Argentina | Facundo Argüello | 173 | 8 | |
 Brazil | André Ghem | 188 | 9 |
The following players received wildcards into the singles main draw:
Patrik Fabian Lukáš Klein Martin Kližan Dominik ŠprochThe following player received entry into the singles main draw as a special exempt:
The following players received entry from the qualifying draw:
The following players received entry as a lucky loser:
See main article: 2016 Poprad-Tatry ATP Challenger Tour – Singles.
Horacio Zeballos def. Gerald Melzer, 6–3, 6–4See main article: 2016 Poprad-Tatry ATP Challenger Tour – Doubles.
Andrey Golubev def. Lukáš Dlouhý / Andrej Martin, 6–2, 5–7, [10–5]
