| Club: | Orlando City B |
| Season: | 2016 |
| League: | USL |
| Mgrtitle: | Head coach |
| Manager: | Anthony Pulis |
| Stadium: | Titan Soccer Complex |
| League Result: | Conference: 8th |
| Cup1: | USL Playoffs |
| Cup1 Result: | Conference Quarterfinals |
| Pattern La1: | _adidasaltwhite |
| Pattern B1: | _OCSC_15h |
| Pattern Ra1: | _adidasaltwhite |
| Pattern Sh1: | _adidaswhite |
| Pattern So1: | _3_stripes_white2 |
| Leftarm1: | 633394 |
| Body1: | FFFFFF |
| Rightarm1: | 633394 |
| Shorts1: | 633394 |
| Socks1: | 633394 |
| Pattern La2: | _whitecuffpiping |
| Pattern B2: | _collarwithbodywhite |
| Pattern Ra2: | _whitecuffpiping |
| Pattern Sh2: | _adidasonwhite |
| Pattern So2: | _3_stripes_on_white2 |
| Leftarm2: | 633394 |
| Body2: | 633394 |
| Rightarm2: | 633394 |
| Shorts2: | 633394 |
| Socks2: | 633394 |
| American: | true |
| Nextseason: | 2017 |
The 2016 Orlando City B season was the club's inaugural year of existence, and their first season in the Eastern Conference of the United Soccer League, the third tier of the United States soccer pyramid.
See main article: 2016 USL season.