The 2016 Open de Nice Côte d'Azur was a men's tennis tournament played on outdoor clay courts. It was the 32nd and final edition of the Open de Nice Côte d'Azur and part of the ATP World Tour 250 series of the 2016 ATP World Tour. It took place at the Nice Lawn Tennis Club in Nice, France, from 15 May until 21 May 2016. The tournament was replaced on the ATP World Tour by the Lyon Open. First-seeded Dominic Thiem won the singles title.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Austria | Dominic Thiem | 15 | 1 | |
 France | Gilles Simon | 18 | 2 | |
| Kevin Anderson | 19 | 3 | ||
| France | Benoît Paire | 21 | 4 | |
| João Sousa | 30 | 5 | ||
 Italy | Fabio Fognini | 31 | 6 | |
| Italy | Andreas Seppi | 42 | 7 | |
| Alexander Zverev | 44 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry using a protected ranking:
The following players received entry from the qualifying draw:
Martin Kližan →replaced by 
Chung Hyeon
Juan Mónaco →replaced by Adrian Mannarino
Tommy Robredo →replaced by Daniel Muñoz de la Nava
Kyle Edmund (left ankle injury)| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
 Colombia | Juan Sebastián Cabal | Colombia | Robert Farah | 44 | 1 | |
| Mate Pavić |  New Zealand | Michael Venus | 80 | 2 | ||
 United States | Eric Butorac | United States | Scott Lipsky | 87 | 3 | |
 Australia | Chris Guccione |  Brazil | André Sá | 111 | 4 |
The following pairs received wildcards into the doubles main draw:
The following pair received entry as alternates:
Diego Schwartzman (left foot injury) Denis Kudla (lower back injury) Dominic Thiem defeated Alexander Zverev, 6–4, 3–6, 6–0 Juan Sebastián Cabal / Robert Farah defeated Mate Pavić / Michael Venus, 4–6, 6–4, [10–8]
