The 2016 Open Sopra Steria de Lyon was a professional tennis tournament played on clay courts. It was the 1st edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Lyon, France, between 6 and 12 June 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Japan | Taro Daniel | 93 | 1 | |
 Spain | Roberto Carballés Baena | 114 | 2 | |
 Italy | Thomas Fabbiano | 117 | 3 | |
 Argentina | Carlos Berlocq | 126 | 4 | |
 Brazil | Thiago Monteiro | 139 | 5 | |
| Argentina | Renzo Olivo | 145 | 6 | |
| Italy | Luca Vanni | 159 | 7 | |
 Belgium | Steve Darcis | 161 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following players received entry as lucky losers:
Clement Geens
Maxime TeixeiraSee main article: 2016 Open Sopra Steria de Lyon – Singles.
Steve Darcis def. Thiago Monteiro 3–6, 6–2, 6–0.See main article: 2016 Open Sopra Steria de Lyon – Doubles.
Grégoire Barrère / Tristan Lamasine def. Jonathan Eysseric / Franko Škugor 2–6, 6–3, [10–6].