The 2016 Open Città della Disfida was a professional tennis tournament played on clay courts. It was the 17th edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Barletta, Italy between 11 and 17 April 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Brazil | Rogério Dutra Silva | 103 | 1 | |
 Spain | Roberto Carballés Baena | 116 | 2 | |
 Czech Republic | Adam Pavlásek | 119 | 3 | |
| Gastão Elias | 122 | 4 | ||
 Slovakia | Andrej Martin | 129 | 5 | |
| Igor Sijsling | 132 | 6 | ||
 Italy | Luca Vanni | 140 | 7 | |
 Sweden | Elias Ymer | 144 | 8 |
The following players received wildcards into the singles main draw:
Julien Benneteau Edoardo Eremin Federico Gaio Gianluca MagerThe following player received entry into the singles main draw with a protected ranking:
The following players received entry from the qualifying draw:
The following player received entry as a lucky loser:
See main article: 2016 Open Città della Disfida – Singles.
Elias Ymer def. Adam Pavlásek, 7–5, 6–4See main article: 2016 Open Città della Disfida – Doubles.
Johan Brunström / Andreas Siljeström def. Flavio Cipolla / Rogério Dutra Silva, 0–6, 6–4, [10–8]