| Date: | 25–31 July |
| Edition: | 31st (men) 2nd (women) |
| Category: | ATP Challenger Tour ITF Women's Circuit |
| Location: | Segovia, Spain |
| Prize Money: | €64,000+H (men) $10,000 (women) |
| Champms: |  Luca Vanni |
| Champws: |  Jessika Ponchet |
| Champmd: |  Purav Raja / Divij Sharan |
| Champwd: |  Charlotte Römer / Sarah-Rebecca Sekulic |
The 2016 Open Castilla y León was a professional tennis tournament played on outdoor hard courts. It was the 31st edition, for men, and 2nd edition, for women, of the tournament and part of the 2016 ATP Challenger Tour and the 2016 ITF Women's Circuit, offering totals of €64,000+H, for men, and $10,000, for women, in prize money. It took place in El Espinar, Segovia, Spain, on 25–31 July 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Spain | Nicolás Almagro | 45 | 1 | |
 Czech Republic | Lukáš Rosol | 79 | 2 | |
 Ukraine | Illya Marchenko | 84 | 3 | |
| Marco Chiudinelli | 129 | 4 | ||
 Bosnia and Herzegovina | Mirza Bašić | 139 | 5 | |
 Poland | Jerzy Janowicz | 149 | 6 | |
| France | Kenny de Schepper | 159 | 7 | |
 Hungary | Márton Fucsovics | 166 | 8 |
The following player received a wildcard into the singles main draw:
Nicolás Almagro Jorge Hernando Ruano
Akira Santillan Ricardo Villacorta-AlonsoThe following players received entry into the singles main draw with a protected ranking:
The following players received entry from the qualifying draw:
Daniel Cox
Alex De Minaur
Viktor Durasovic Jaume Pla Malfeito| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Slovakia | Michaela Hončová | 365 | 1 | |
| Italy | Giulia Gatto-Monticone | 484 | 2 | |
| France | Jessika Ponchet | 547 | 3 | |
 Romania | Ioana Loredana Roșca | 565 | 4 | |
| Italy | Bianca Turati | 611 | 5 | |
| Spain | María José Luque Moreno | 621 | 6 | |
| Australia | Isabelle Wallace | 651 | 7 | |
| Inês Murta | 716 | 8 |
The following player received a wildcard into the singles main draw:
Arabela Fernández Rabener Michaela Hončová Claudia Hoste Ferrer Ana Román DomínguezThe following players received entry from the qualifying draw:
Lucy Brown Alba Carrillo Marín Yésica de Lucas Jazzamay Drew Ángela Fita Boluda Lucía Marzal Martínez Yuriko Miyazaki Giorgia PintoThe following player received entry by a lucky loser spot:
Laura SainsburySee main article: 2016 Open Castilla y León – Men's singles.
Luca Vanni def. Illya Marchenko, 6–4, 3–6, 6–3. Jessika Ponchet def. Rocío de la Torre Sánchez, 6–4, 6–2See main article: 2016 Open Castilla y León – Men's doubles.
Purav Raja / Divij Sharan def. Quino Muñoz / Akira Santillan, 6–3, 4–6, [10–8] Charlotte Römer / Sarah-Rebecca Sekulic def. Jessika Ponchet / Ioana Loredana Roșca, 6–2, 7–6(7–4)