| Competition: | Nigeria Premier League |
| Season: | 2016 |
| Winners: | Enugu RangersGUI |
| Relegated: | Giwa Heartland Ikorodu United Warri Wolves |
| Continentalcup1: | Champions League |
| Continentalcup1 Qualifiers: | Enugu Rangers Rivers United |
| Continentalcup2: | Confederation Cup |
| Continentalcup2 Qualifiers: | Wikki Tourist |
| Matches: | 342 |
| Total Goals: | 734 |
| League Topscorer: | Godwin Obaje (18) |
| Biggest Home Win: | Kano Pillars 6-0 Shooting Stars (24 April 2016) |
| Biggest Away Win: | El Kanemi Warriors 1-3 Akwa United (30 March 2016) |
| Longest Wins: | Plateau United Wikki Tourist (4) |
| Longest Unbeaten: | Abia Warriors (10) |
| Longest Winless: | Ikorodu United (10) |
| Longest Losses: | Ikorodu United (5) |
| Highest Attendance: | 40,000 El Kanemi at Rangers International |
| Average Attendance: | 8,600 |
| Prevseason: | 2015 |
| Nextseason: | 2017 |
| Updated: | 2 October 2016 |
The 2016 Nigeria Professional Football League (referred to as the Nigerian Glo premier league for sponsorship reasons) is the 45th season of the Nigeria Premier League, the top Nigerian professional league for association football clubs, since its establishment in 1972, and the 26th since the rebranding of the league as the "Professional League".
Enugu Rangers won their seventh league title and first since 1984. Giwa, Heartland, Ikorodu United and Warri Wolves finished as the bottom four teams in the standings and will be relegated to Nigeria National League for the 2017 season.
A total of 20 teams will contest the league including 16 teams from the previous season and four teams promoted from the Nigeria National League. The four promoted teams replace Bayelsa United, Kwara United, Sharks and Taraba who were all relegated to the National League at the end of the previous season