The 2016 Nielsen Pro Tennis Championship is a professional tennis tournament played on hard courts. It is the 25th edition of the tournament which is part of the 2016 ATP Challenger Tour. It will take place in Winnetka, Illinois, between 4 and 10 July 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Japan | Yoshihito Nishioka | 122 | 1 | |
| Tim Smyczek | 125 | 2 | ||
| Austin Krajicek | 132 | 3 | ||
| Marco Chiudinelli | 138 | 4 | ||
| Japan | Go Soeda | 156 | 5 | |
| Peter Gojowczyk | 157 | 6 | ||
 Australia | John-Patrick Smith | 160 | 7 | |
| Frances Tiafoe | 167 | 8 |
The following players received wildcards into the singles main draw:
Tom Fawcett Jared Hiltzik Mackenzie McDonald Alex RybakovThe following player received entry into the singles main draw with a protected ranking:
The following players received entry from the qualifying draw:
Alex Kuznetsov Dennis Nevolo Ryan ShaneSee main article: 2016 Nielsen Pro Tennis Championship – singles.
Yoshihito Nishioka def. Frances Tiafoe, 6–3, 6–2See main article: 2016 Nielsen Pro Tennis Championship – doubles.
Stefan Kozlov / John-Patrick Smith def. Sekou Bangoura / 
David O'Hare, 6–3, 6–3