| Year: | 2016 |
| Team: | Nicholls State Colonels |
| Sport: | football |
| Conference: | Southland Conference |
| Short Conf: | Southland |
| Record: | 5–6 |
| Conf Record: | 5–4 |
| Head Coach: | Tim Rebowe |
| Hc Year: | 2nd |
| Off Coach: | Rob Christophel |
| Oc Year: | 2nd |
| Off Scheme: | Multiple spread |
| Def Coach: | Tommy Rybacki |
| Dc Year: | 2nd |
| Def Scheme: | Multiple |
| Stadium: | John L. Guidry Stadium |
The 2016 Nicholls State Colonels football team represented Nicholls State University as a member of the Southland Conference during the 2016 NCAA Division I FCS football season. Led by second-year head coach Tim Rebowe, the Colonels compiled an overall record of 5–6 with a mark of 5–4 in conference play, tying for fourth place in the Southland. Nicholls State played home games at John L. Guidry Stadium in Thibodaux, Louisiana.
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