| Date: | 2–8 May |
| Edition: | 5th |
| Category: | ITF Women's Circuit |
| Location: | Tunis, Tunisia |
| Prize Money: | $50,000 |
| Champs: |  Ons Jabeur |
| Champd: |  Arina Rodionova /  Valeriya Strakhova |
The 2016 Nana Trophy was a professional tennis tournament played on outdoor clay courts. It was the fifth edition of the tournament and part of the 2016 ITF Women's Circuit, offering a total of $50,000 in prize money. It took place in Tunis, Tunisia, on 2–8 May 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Russian Federation | Evgeniya Rodina | 119 | 2 | |
| Romina Oprandi | 137 | 2 | ||
 Belgium | Ysaline Bonaventure | 147 | 3 | |
| Russian Federation | Irina Khromacheva | 157 | 4 | |
 Czech Republic | Tereza Smitková | 161 | 5 | |
| Lesley Kerkhove | 174 | 6 | ||
 Turkey | İpek Soylu | 176 | 7 | |
 France | Myrtille Georges | 202 | 8 |
The following players received wildcards into the singles main draw:
Fiona Codino
Dea Herdželaš Yasmine Mansouri
Sandra SamirThe following players received entry from the qualifying draw:
See main article: 2016 Nana Trophy – Singles.
Ons Jabeur def. Romina Oprandi, 1–6, 6–2, 6–2See main article: 2016 Nana Trophy – Doubles.
Arina Rodionova / Valeriya Strakhova def. Irina Khromacheva / İpek Soylu, 6–1, 6–2