| Champ: |  Simona Halep |
| Runner: |  Dominika Cibulková |
| Score: | 6–2, 6–4 |
| Before Name: | Mutua Madrid Open – Women's singles |
| After Name: | Mutua Madrid Open – Women's singles |
See main article: 2016 Mutua Madrid Open.
Simona Halep defeated Dominika Cibulková in the final, 6–2, 6–4 to win the women's singles tennis title at the 2016 Madrid Open.
Petra Kvitová was the defending champion, but lost in the third round to Daria Gavrilova.